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Peptide Institute fluorogenic substrate ac thz tle leu glnmca
Fluorogenic Substrate Ac Thz Tle Leu Glnmca, supplied by Peptide Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tle/acetyl+aminomethylcoumarin+asp+fluorogenic+glu+substrate+valasp/pm41107840-70-8-11
Average 86 stars, based on 1 article reviews
fluorogenic substrate ac thz tle leu glnmca - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Inhibitory effect of [6]-shogaol against 3CLpro activity and SARS-CoV-2 infection
Article Snippet: 3CLpro and PLpro assays were performed using the fluorogenic substrate Ac-Thz-Tle-Leu-Gln-MCA (PEPTIDE Institute Inc., Osaka, Japan) and Z-Arg-Leu-Arg-Gly-Gly-MCA (PEPTIDE Institute Inc).

Article Title: Inhibitory effect of [6]-shogaol against 3CLpro activity and SARS-CoV-2 infection.
Article Snippet: 3CLpro and PLpro assays were performed using the fluorogenic substrate Ac-Thz-Tle-Leu-GlnMCA (PEPTIDE Institute Inc., Osaka, Japan) and Z-ArgLeu-Arg-Gly-Gly-MCA (PEPTIDE Institute Inc).

Article Title: Biochemical Screening of Phytochemicals and Identification of Scopoletin as a Potential Inhibitor of SARS-CoV-2 M pro , Revealing Its Biophysical Impact on Structural Stability
Article Snippet: In our assay, we have utilized Ac-Abu-Tle-Leu-Gln-MCA (Cat/Code No.: 3250-v; Peptide Institute, Inc., Osaka, Japan, https://www.peptide.co.jp/en/ (accessed on 12 July 2023)), a specific fluorogenic probe/substrate similar to Ac-Abu-Tle-Leu-Gln-MCA, as described by Rut, Groborz et al. [ , ].

Article Title: Supersulphides provide airway protection in viral and chronic lung diseases
Article Snippet: Each reaction mixture for PL pro , 3CL pro , papain, or cathepsin B was reacted with corresponding MCA substrate (10 μM; PEPTIDE Institute, Inc.), i.e., Z-Leu-Arg-Gly-Gly-MCA, Ac-Abu-Tle-Leu-Gln-MCA, Bz-Arg-MCA, or Z-Arg-Arg-MCA, respectively, in 50 mM HEPES buffer (pH 7.5) at 37 °C.

Article Title: Study of key residues in MERS-CoV and SARS-CoV-2 main proteases for resistance against clinically applied inhibitors nirmatrelvir and ensitrelvir
Article Snippet: Then, 10 μl of the fluorogenic substrate Ac-Abu-Tle-Leu-Gln-MCA (Peptide Institute, Japan) were added and incubated over night at room temperature.

Article Title: Biochemical Screening of Phytochemicals and Identification of Scopoletin as a Potential Inhibitor of SARS-CoV-2 M pro , Revealing Its Biophysical Impact on Structural Stability.
Article Snippet: In our assay, we have utilized Ac-Abu-Tle-Leu-GlnMCA (Cat/Code No.: 3250-v; Peptide Institute, Inc., Osaka, Japan, https://www.peptide. co.jp/en/ (accessed on 12 July 2023)), a specific fluorogenic probe/substrate similar to Ac-Abu-Tle-Leu-Gln-MCA, as described by Rut, Groborz et al. [48,49].

Article Title: Discovery of Chlorofluoroacetamide-Based Covalent Inhibitors for Severe Acute Respiratory Syndrome Coronavirus 2 3CL Protease.
Article Snippet: The coronavirus disease 2019 (COVID-19) pandemic has necessitated the development of antiviral agents against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).. 3C-like protease (3CLpro) is a promising target for COVID-19 treatment.. Here, we report a new class of covalent inhibitors of 3CLpro that possess chlorofluoroacetamide (CFA) as a cysteinereactive warhead.

Activity Assay:

Article Title: Development of a Biosafety Level 1 Cellular Assay for Identifying Small-Molecule Antivirals Targeting the Main Protease of SARS-CoV-2: Evaluation of Cellular Activity of GC376, Boceprevir, Carmofur, Ebselen, and Selenoneine
Article Snippet: .. The effect of selenoneine on Mpro’s protease activity was evaluated using a fluorogenic peptide substrate, Ac-Abu-Tle-Leu-Gln-MCA (Peptide Institute, Osaka, Japan, Cat. No. 3250-v). .. The reaction mixture contained 50 mM Tris-HCl (pH 7.4), 10 μM substrate, 2 μg/mL Mpro, and varying concentrations of ebselen, selenoneine, or ergothioneine (Nacalai Tesque, Kyoto, Japan).



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